Telomere Science Library

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about the Nobel-Prize Winning Science of Telomere Biology

Synthesis and Evaluation of DNA Based Quantum Dot Fluorescence In Situ Hybridization (FISH) Probe for Telomere Detection.

Authors: Youngseon Y. Choi, Jair Lage JL. De Siqueira Neto, Carolina C. Moraes, Lucio L. Freitas Jr, Rita R. Song
Published: 09/10/2015, Journal of nanoscience and nanotechnology


Efficient oligonucleotide probe design and synthesis based on polymer-coated CdSe/ZnS quantum dot (QD) is demonstrated for detection of telomeres in human monocyte and Leishmania major, a protozoan pathogenic parasite. The highly photoluminescent polymer-coated QDs conjugated with various length of telomere probe sequences were prepared via carbodiimide chemistry and characterized. Specific detection of telomere was observed when DNA sequence was (CCCAAT)n (n = 5 or 3) probe sequence, rather than (GGGTTA)n (n = 3, 5, 8). The sensitivity and specificity were comparable with commercially available PNA probe for human telomere detection.