Telomere Science Library

Publications, Presentations, and Videos
about the Nobel-Prize Winning Science of Telomere Biology

Comparison between southern blots and qPCR analysis of leukocyte telomere length in the health ABC study.

Authors: Clara C CC. Elbers, Melissa E ME. Garcia, Masayuki M. Kimura, Steven R SR. Cummings, Mike A MA. Nalls, Anne B AB. Newman, Vicki V. Park, Jason L JL. Sanders, Gregory J GJ. Tranah, Sarah A SA. Tishkoff, Tamara B TB. Harris, Abraham A. Aviv
Published: 08/14/2013, The journals of gerontology. Series A, Biological sciences and medical sciences


Only a few studies, primarily limited to small samples, have examined the relationship between leukocyte telomere length (LTL) data generated by Southern blots, expressed in kilobases, versus quantitative PCR data, expressed in the telomere product/a single gene product (T/S). In the present study, we compared LTL data generated by the two methods in 681 elderly participants (50% African Americans, 50% of European origin, 49.2% women, mean age 73.7±2.9 years) in the Health Aging and Body Composition Study. The correlation between the data generated by the two methods was modest (R (2) = .27). Both methods captured the age effect on LTL and the longer LTL in women than in men. However, only the Southern blot method showed a significantly longer LTL in African Americans than in European decent individuals, which might be attributed to the larger measurement error of the quantitative PCR-based method than the Southern blots.

PubMed Full Text