Telomere Science Library

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about the Nobel-Prize Winning Science of Telomere Biology

Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH.

Authors: Reelina R. Basu, Lan-Tian LT. Lai, Zhenyu Z. Meng, Jun J. Wu, Fangwei F. Shao, Li-Feng LF. Zhang
Published: 09/16/2014, PloS one


Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long non-coding RNAs being identified in the current research. With probes either chemically or biologically synthesized, we demonstrate that the method can be applied to study a wide range of RNA and DNA targets at the single-cell and single-molecule level in cellular contexts.

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