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Telomere-mediated chromosomal truncation in Aspergillus oryzae.

Authors: Sawaki S. Tada, Hikaru H. Ohkuchi, Mayumi M. Matsushita-Morita, Ikuyo I. Furukawa, Ryota R. Hattori, Satoshi S. Suzuki, Yutaka Y. Kashiwagi, Ken-Ichi K. Kusumoto
Published: 07/14/2014, Journal of bioscience and bioengineering


We truncated the short arm of chromosome 3 to delete the aflatoxin biosynthesis gene homolog cluster using telomeric repeats in Aspergillus oryzae. The predicted deletion was confirmed by Southern blot analyses. This telomere-mediated chromosomal truncation method enables the development of an artificial chromosome in A. oryzae.

Copyright © 2014 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
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